Read e-book online An Introduction to Genetic Engineering (Studies in Biology) PDF

By Dr Desmond S. T. Nicholl

ISBN-10: 0521004713

ISBN-13: 9780521004718

Des Nicholl provides a brand new, absolutely revised, and multiplied version of his well known undergraduate-level textbook. The publication keeps some of the good points of the unique variation and nonetheless bargains a concise technical creation to the topic of genetic engineering. it really is divided into 3 major sections: simple molecular biology, equipment of gene manipulation, and sleek functions of genetic engineering. purposes lined within the booklet comprise genomics, protein engineering, gene remedy, cloning, transgenic animals and crops, and bioethics. An advent to Genetic Engineering is key interpreting for undergraduate scholars of biotechnology, genetics, molecular biology, and biochemistry.

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One common technique is SDS–PAGE, in which the detergent SDS (sodium dodecyl sulphate) is used to denature multisubunit proteins and cover the protein molecules with negative charges. In this way the inherent charge of the protein is masked, and the charge/mass ratio becomes constant. Thus proteins can be separated according to their size in a similar way to DNA molecules. 6 DNA sequencing The ability to determine the sequence of bases in DNA is a central part of modern molecular biology, and provides what might be considered as the ultimate structural information.

The double digests enable a map to be drawn up, and the triple digest confirms this. Construction of the map is outlined in Fig. 4. 2. Digestion of a 15 kb DNA fragment with three restriction enzymes BamHI EcoRI Pstl 14 1 12 3 8 7 BamHI ϩ EcoRI BamHI ϩ PstI EcoRI ϩ PstI 11 3 1 8 6 1 7 5 3 BamHI ϩ EcoRI ϩ PstI 6 5 3 1 Note: Data shown are lengths (in kb) of fragments that are produced on digestion of a 15 kb DNA fragment with the enzymes BamHI, EcoRI and PstI. Single, double and triple digests were carried out as indicated.

The next step is a chemical modification of the bases in the DNA strand. This is done in a series of four or five reactions with different specificities, and the reaction conditions are chosen so that, on average, only one modification will be introduced into each copy of the DNA molecule. The modified bases are then removed from their sugar groups and the strands cleaved at these positions using the chemical piperidine. The theory is that, given the large number of molecules and the different reactions, this process will produce a set of fragments which terminate at different bases and differ in length by one nucleotide.

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An Introduction to Genetic Engineering (Studies in Biology) by Dr Desmond S. T. Nicholl


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